Home | FAQ's

Proteomics

Services on the Agilent platform

The proteomics facility at ILS bioservices has state-of-the-art technologies available to meet conventional and customized needs of academic and industry researchers. Our services include Cloning, expression, mutagenesis, protein identification, sequencing, analytical and characterization contract services. Dedicated modules of proteomic services from designing expression systems to sample preparation and separation by multiple techniques such as chromatography, SPE, gel-based techniques, HPLC, LC-MS are routinely offered to meet industry's research needs. All the experiments are conducted in dedicated CLASS 10000 clean room laboratory clusters to ensure internationally acceptable results.

Cloning and expression services:

We offer conventional and customized vector design and development for various expression systems such as E.coli, yeast and mammalian cells using state of the art protocols. Services include:

  • Cloning of the DNA in our standard vector
  • Cloning of the DNA in the expression vector of your choice
  • Optimized protein expression for E.coli, yeast and mammalian cells
  • Design and construction of cell specific promoter containing vectors
  • Inducible vector systems for expressing toxic proteins
  • Mutated protein expression and epitope modifications
  • Organ based and cell based Protein expression profiling
  • Phosphorylation and functional studies
  • Fusion tag design for protein purification
  • Segregated expression of protein subunits
  • Secretory protein expression
  • Large scale production of biomolecules in shortest time
  • Biostability, serum binding and solubility studies

Protein purification and characterization services:

  • Assay system design and development
  • Customized protein purification platforms for crystallography studies.
  • Functional assay studies
  • Small Scale protein purification for proof of concept studies
  • Receptor binding studies for hormones and enzymes
  • Enzyme Kinase assays.
  • Picogram level protein analysis using Bioanalyzer
  • Certificate of analysis of biomolecule for quality determination using Octet RED, electrophoresis, western blot, IHC, 2-D Electrophoresis, Nanodrop based quantitation assays, Absorption Spectra, ELISA, Activity analysis, RP-HPLC.

Protein Biomarker Discovery:

Fast and accurate results in protein Biomarker discovery in a cost effective manner, with:

  • Antibody Microarrays
  • ELISA, ELISPOT Assays
  • Immunohistochemistry of cell and paraffin embedded organs and tissues
  • Whole Mouse embryo staining for developmental biomarker profiling
  • Octet RED based antibody quantitation, epitope mapping
  • Clonal selection and Hybridoma characterization for antibodies
  • Two-Dimensional Gel Electrophoresis, followed by MALDI-TOF/TOF

Small molecule screening studies:

  • High Throughput Assay Development-in-vitro and ex-vivo (CAM based)
  • GPCR and Kinase profiling for therapeutic & diagnostic discovery
  • Octet RED (biosensor) based rapid assay out for protein biomarker discovery, protein-protein interaction studies etc.
  • Mammalian and Yeast based assay system designs for small molecule screening
  • Proprietary platforms for iNOS, Interleukins, HDAC inhibitors and immunostimulatory molecules
  • 3-D cell system for Cancer studies
  • CAM studies for endothelial cell development and angiogenesis
  • Stability and half life studies of small molecules
  • Caco-2 and PAMPA based drug transport and absorption studies for therapeutic small molecules
  • Drug Compound Screening with real time kinetics report.

Adenoviral Expression Systems:

  • Customized protein expression using Adeno virus vector systems for guaranteed protein expression in "difficult to transfect" primary cell lines
  • Adenoviral siRNA vector systems for 100% knockdown of candidate mRNA expression
  • Cell specific Promoter driven Adenoviruses for restricted expression
  • Epigenetic expression of proteins under inducible promoters
  • Construction of clinically relevant Adeno-Associate virus (AAV) based expression systems
  • Modified Lentiviral expression cassettes.
  • Full expression panel design services- The package contains, cloning, expression of candidate proteins with (N-or C- terminal) and without tags, adenoviral expression, certified antibody and IHC documentation

For more details please Click here for the list of arrays.